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. 2018 Apr 30;9:432. doi: 10.3389/fphar.2018.00432

FIGURE 6.

FIGURE 6

The effects of OPD′ on DU145 prostate cancer cells in vitro and in vivo. (A) DU145 cells were exposed to various concentrations of OPD′ for 6 h, then the protein levels of RIPK1, C-PIPK1, caspase-8, and C-caspase 8 were examined by Western blotting. n = 3 independent experiments; this panel represents one of the replicates. (B) After pre-treatment with 10 μM Nec-1 for 2 h, the cells were exposed to various concentrations of OPD′ for 18 h, followed by the Annexin V-FITC/PI assay. Data are presented as the means ± SD; n = 3 independent experiments. ∗p < 0.05 vs. 2.5 μM OPD′ alone. (C) The tumors were measured every 3 days to assess their growth. OPD′ was injected i.p. at doses of 2.5 and 5.0 mg/kg/d, 5 d/wk for 18 days. Each group comprised eight mice. One of the mice in the control group died on Day 12. ∗p < 0.05 vs. the control group. (D) Representative tumors were weighed and photographed at the end of the experiment. (E) The weight distribution of the tumors at the end of the experiment. ∗p < 0.05 vs. the control group.