Figure 1.

Generation of TAP–14-3-3ζ MEFs to elucidate the 14-3-3ζ interactome. A, schematic overview of generation and use of TAP–14-3-3ζ MEFs to determine the 14-3-3ζ interactome during adipogenesis. B and C, verification of TAP–14-3-3ζ MEF adipogenesis by Oil Red-O incorporation, 7 days after induction (B), or Pparg mRNA expression by quantitative PCR (C), 2 days following induction (UD, undifferentiated cells; representative of n = 4 independent experiments; *, p < 0.05 when compared with -MDI; bar graphs represent means ± S.D.). Rosi., rosiglitazone. D, String-db (17) was used to visualize and cluster proteins according to their biological function, resulting in three distinct clusters: RNA splicing/processing factors, components of the ribosomal complex, and components of actin/tubulin network.