Known regulators of adipogenesis can be found within the 14-3-3ζ interactome.
A, 3T3-L1 cells were transfected with a control siRNA (siCon) or two independent siRNAs (siRNAs 1 and 2) against target mRNA, and knockdown efficiency was measured by quantitative PCR (n = 4 per group; *, p < 0.05 when compared with siCon-transfected cells; bar graphs represent means ± S.D.). B and C, transient knockdown by siRNA of previously identified regulators of adipogenesis or those associated with the development of obesity was used to examine their contributions to adipocyte differentiation (+MDI), as assessed by visualization of Oil Red-O incorporation (B), absorbance (490 nm, C) (*, p < 0.05 when compared with siCon + MDI; bar graphs represent means ± S.D.). D–F, measurement of total Pparg mRNA levels (D) or Pparγ protein abundance (E and F) in siCon or siRNA-transfected 3T3-L1 cells induced to differentiate (+MDI) for 48 h (n = 4 per group; *, p < 0.05 when compared with siCon-transfected differentiated cells; bar graphs represent means ± S.D.).