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. 2018 Mar 12;293(18):6736–6750. doi: 10.1074/jbc.M117.816272

Figure 5.

Figure 5.

RNA splicing proteins are required for 3T3-L1 adipogenesis. A, 3T3-L1 cells were transfected with a siCon or two independent siRNAs (siRNAs 1 and 2) against target mRNA, and knockdown efficiency was measured by quantitative PCR (n = 4 per group; *, p < 0.05; bar graphs represent means ± S.D.). B–D, transient knockdown by siRNA was used to examine the contributions of RNA-splicing factors to adipocyte differentiation (+MDI), as assessed by visualization of Oil Red-O incorporation (B), absorbance associated with Oil Red-O (490 nm, C), or Pparg mRNA expression (D) (n = 4 per group; *, p < 0.05 when compared with differentiated, siCon-transfected cells; bar graphs represent means ± S.D.).