Table 6.
Kinetic parameters from processivity assays
| Incorporation |
kpol |
koff |
||
|---|---|---|---|---|
| DNA | RNA | DNA | RNA | |
| s−1 | s−1 | |||
| First | 14.0 ± 0.9 | 14.1 ± 0.7 | 4.5 ± 1.2 | 4.5 ± 0.8 |
| Second | 49.9 ± 7.1 | 16.2 ± 1.1 | 11.0 ± 3.6 | 4.0 ± 0.8 |
| Third | 11.2 ± 1.3 | 11.4 ± 1.0 | 4.2 ± 1.6 | 2.1 ± 0.7 |
| Fourth | 19.3 ± 4.5 | 8.3 ± 1.0 | 5.8 ± 3.7 | 0.9 ± 0.8 |
| Fifth | 38.0 ± 16.8 | 28.1 ± 8.9 | 6.5 ± 7.6 | 1.8 ± 2.5 |
| Sixth | 14.6 ± 4.8 | 20.6 ± 6.2 | 2.4 ± 3.8 | 1.8 ± 2.5 |
| Seventh | 14.1 ± 7.4 | 24.6 ± 9.9 | 1.2 ± 5.0 | 2.0 ± 3.4 |
| Average (second to fourth) | 26.8 | 12.0 | 7.0 | 2.3 |
| Average (fifth to seventh) | 22.2 | 24.4 | 3.4 | 1.9 |