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. 2018 Mar 16;293(18):6693–6706. doi: 10.1074/jbc.RA118.001689

Figure 4.

Figure 4.

Wnt7a deficiency decreased the invasiveness of 5637 HMI and T24 cells. A, expression level of Wnt7a and constitutively active β-catenin in UBC cell lines. B and C, Wnt7a mRNA expression levels in 5637 HMI (B) and T24 cells (C) transfected with control (siCTL) and two Wnt7a siRNAs (siWnt7a-1 and siWnt7a-2) by qRT-PCR. D, Western blotting showed EMT and invasiveness-associated protein expression in 5637 HMI and T24 cells transfected with siCTL, siWnt7a-1, and -2. E, transwell assay showed the invasive capacities of 5637 HMI and T24 cells followed by siCTL and Wnt7a siRNA treatment. The upper photograph are representative fields of staining invaded cells 16 h after seeding. Scale bar, 100 μm. The lower graphs indicated the counting number of invaded cells. The assays were performed in three independent biological replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001.