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. 2018 Mar 14;293(18):6925–6941. doi: 10.1074/jbc.RA118.002566

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© 2018 Peoples et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — Effects of adrenergic deficiency on GAPDH activity and protein concentrations. A, steady-state metabolites involved in glycolysis in E11.5 adrenergic hormone-deficient hearts compared with controls (dotted line). Schematic representation of glycolysis pathway provided as reference. B, GAPDH activity in adrenergic hormone-competent (●) and adrenergic hormone-deficient (□) embryonic hearts, expressed as milliunits of enzyme per ml of sample and NADH produced per min, respectively. Milliunits/ml are calculated as ((nmol of NADH)/(min)(ml of sample)). C and D, GAPDH protein concentrations (36 kDa) in adrenergic hormone-deficient embryonic trunks (□) compared with controls (●), normalized to β-actin. The β-actin panel is re-used as a representative image of six replicated experiments probing for GAPDH, G-6-PDH, and PDH. Numerical values below the x axes refer to the number (n) of samples analyzed. Student's t test was used to compare means between competent and deficient groups. *, p < 0.05.