Figure 4.
A, current–voltage relationship of reconstituted OmpF channels under symmetrical 30 mm KCl (cis/trans) bi-ionic conditions (control) and under tri-ionic conditions with 80 mm ampicillin (cis) at pH 8 (see Table 1), Vrev = 21.5 ±4.8. B, calculated current–voltage relationship for a single trimeric OmpF channel (i(Σ)) with the cis/trans tri-ionic concentrations given in Table 1 and the separated currents carried by K+ or Cl− ions (i(KCl)) and iampicillin = (i(Σ)) − (i(KCl)) (for more details see supporting data). Conditions: 30 mm KCl, buffered with 10 mm MES, pH 6.0. OmpF and ampicillin was added to cis = GND side.