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A, current–voltage relationship of reconstituted OmpF under symmetrical 30 mm KCl (cis/trans), pH 6, buffered with 10 mm MES, bi-ionic conditions (control), and under tri-ionic, pH 6, conditions with 80 mm benzylpenicillin (cis, see Table 1). B, calculated current–voltage relationship for a single OmpF pore bathed in 100 mm KCl symmetrical (cis/trans) and 10 μm (theoretical) benzylpenicillin (cis) (for details see supporting data).
