Figure 4.

Binding by the light chain of YES8c. A, interaction footprints in the light chain of YES8c to Fc (left panel) and the 90° rotated view (right panel). The contacting area of the light chain to Fc is shown in green. The other surface of the light chain is shown in pale yellow. Fc is shown as a magenta schematic model. B, binding of mutants in the light chain of YES8c to the immobilized IgG1 by ELISA. Data show mean ± S.D. (error bars) from triplicates from one representative experiment of two independent experiments with similar results.