Figure 6.

The YES8c sites relative to the increase in binding affinity for IgG1-Fc. A, the residues (H-Thr56 and H-Asn58) in CDR-H2 located near the acidic residues on Fc (Glu³⁴⁵ and Glu⁴³⁰). B, the residues (L-Gln27 and L-Ser27A) in CDR-L1. C, plot of equilibrium binding responses against concentration of YES8c-Fab mutants for the sensorgrams shown in Fig. S11, B–E. The solid line represents a nonlinear fit of the 1:1 binding model for H-T56K, H-N58K, L-Q27E, and L-S27AN. The K_d values are indicated. RU, resonance unit.