Figure 2.

Mammalian myosin II tail fragments form ordered filaments. A, schematic showing the mammalian myosin IIs and constructs derived from them. Highlight, regions necessary for filament formation (assembly competence domain and nonhelical tailpiece); red barrels, mCherry. B, representative negative-stain EM images of human mCherry-IIA, human mCherry-IIB, and mouse mCherry-IIC filaments. IIA was assembled at 25 mm NaCl, IIB and IIC were assembled at 150 mm NaCl. Images were uniformly adjusted for brightness/contrast and γ to enhance visibility.