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. 2018 Mar 25;293(18):6647–6658. doi: 10.1074/jbc.RA118.002543

Figure 6.

Figure 6.

ENaCs containing the δ-subunit are insensitive to activation by extracellular proteases. a, representative IM traces of oocytes expressing αβγ-ENaC. Proteolytic channel activation was determined by assessing amiloride-sensitive current fractions (ΔIami) before and after application of chymotrypsin (CT; 2 μg/ml) together with amiloride (a). Current traces of oocytes previously incubated with furin inhibitor I (40 μm) are depicted as dotted gray lines. b, comparison of ΔIami before and after application of chymotrypsin in oocytes incubated with (+) or without (−) furin inhibitor I (one-way ANOVA; F = 15.31, p < 0.0001; Tukey's multiple comparison test). c, comparison of -fold activation in ΔIami between experimental groups (chymotrypsin +) and control groups (chymotrypsin −) (one-way ANOVA; F = 44.4, p < 0.0001; Tukey's multiple comparison test). d–f, IM traces (d) and statistical evaluation of changes in ΔIami in oocytes expressing δβγ-ENaC. Data presentation is equivalent to a–c. e, Kruskal–Wallis test, p = 0.376; Dunn's multiple-comparison test. f, one-way ANOVA; F = 1.393, p = 0.2598; Tukey's multiple-comparison test. Lines and error bars, mean and S.E.