Figure 6.

Composition of PLs of hepatic LDs by untargeted lipidomics. Male mice (n = 5–7/group, 14 weeks old) of the indicated genotype were fed a HSD for 4 weeks and refed for 4 h before tissues were collected. Four PL classes (PCs, PEs, PSs, and PGs) were analyzed by direct infusion lipidomics (see “Experimental procedures”). The total content of each PL class was calculated by summing all unambiguous signals obtained in negative mode. Individual PLs were identified by their negative ion with the MS2 of the vLCPUFA. Similar results were observed in positive mode. All values were normalized to the sum of the positive and negative mode signal, where the majority of the signal was TGs. Analysis was repeated once with similar results. Error bars, S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.