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Characterization of the collagen content according to the van Gieson staining and the calcium content using Alizarin Red staining on days 1, 3, 5, and 7. Collagen appeared as early as day 1 and was seen abundantly present until day 7. Calcium emerged on day 3 and was seen until day 7. Collagen and calcium are shown with red and yellow arrows, respectively (40X magnification).
