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Characterization of the co-culture of peripheral blood mononuclear cells with hFOB 1.19 at the ratios of 1 OC: 1 OB, 1 OC: 2 OBs, 1 OC: 4 OBs, and 2 OCs: 1 OB, respectively. The ratio of 1 OC: 2 OBs was chosen as the best ratio because both OBs and OCs were viable and TRAP was moderately expressed. OBs, OCs, and TRAP are shown with red, blue, and yellow arrows, correspondingly (40X magnification).
