Table 1.
Characteristics of enzymatic extract containing lignin peroxidase (LiP) from Pleurotus ostreatus PLO9 and Ganoderma lucidum GRM117.
| Purification steps | Total volume (mL) | Total LiP activity (U mL−1) | Total protein content (mg mL−1) | LiP specific activity (U mg−1) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|
| Pleurotus ostreatus PLO9 | ||||||
| Crude enzymatic extract | 123 | 10602.68 | 0.34 | 30623.93 | 1 | 100 |
| (NH4)2SO4 precipitation | 37 | 10556.27 | 0.25 | 41283.92 | 1.3 | 100 |
| DEAE-cellulose | 25 | 4247.3118 | 0.063 | 66686.76 | 1.6 | 40 |
| Ganoderma lucidum GRM117 | ||||||
| Crude enzymatic extract | 120 | 9419.35 | 0.13 | 69814.04 | 1 | 100 |
| (NH4)2SO4 precipitation | 21 | 7715.05 | 0.13 | 56836.99 | 0.8 | 82 |
| DEAE-cellulose | 25 | 1442.65 | 0.019 | 75424.97 | 1.3 | 15 |