Read depth coverage (blue, y-axis) in 10 Kb blocks for reads mapped to L. braziliensis M2904 chromosome 34 (x-axis) for nine Viannia isolates. The black horizontal line is the median chromosome 34 coverage. L. panamensis PSC-1 (a) and L. shawi M8408 (d) showed a 3′ jump in coverage (green) consistent with an amplification of inverted repeats that could form a linear minichromosome. In addition, this pair shared a 45 Kb amplification (pink) also found in the L. braziliensis M2904 control (g), L. naiffi M5533 (b), L. panamensis WR120 (e), L. peruviana LEM1537 (h), L. peruviana PAB-4377 (c) and L. guyanensis M4147 (f). This was absent in L. lainsoni M6426 (i).