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Virulence of influenza A/Anhui/1/2013 (H7N9) in BALB/c mice. Female BALB/c mice (n = 8 per group) were intranasally inoculated with serial dilutions of the A/Anhui/1/2013 (H7N9) influenza virus on day 0 and survival was monitored for 14 days. Kaplan–Meyer survival curve (A) and mean body weight change is depicted (B). The MLD50 was calculated using the Reed–Muench method.
