Figure 4.

Bacillus Calmette–Guérin (BCG) vaccination does not protect mice during H7N9 influenza infection. Female BALB/c mice were challenged with a dose of 4MLD₅₀ influenza A/Anhui/1/2013 (H7N9) on day 0. Mice received 750 µg BCG or the vehicle control (phosphate buffered saline) i.v. on day −7. Kaplan–Meyer survival curve (A) and mean body weight change (B) are depicted. Survival proportion in the BCG treated group was statistically analyzed using a Fisher’s exact two-sided test. Survival time was statistically analyzed using a Mantel and Cox log-rank test. The effect of BCG treatment on body weight was analyzed by comparing the area under curve of treatment groups with vehicle and was statistically analyzed using a two-way ANOVA with Bonferroni correction. Data are shown as mean ± SEM, n = 8 per group.