Figure 1.

Experimental study design. Dendritic cells (DCs) and natural killer (NK) cells were kept in single cultures, or in cross talk cultures consisting of NK cells and DCs from the same donor at a 1:1 ratio and exposed to 1 μg/ml F-HIV, complement-opsonized HIV (C-HIV), complement- and antibody opsonized HIV (CI-HIV), or mock treated for 24 h. Flow cytometry was performed to assess expression of activation markers on the cells, and the pathways activated were assessed by RNAseq. The concentration of cytokines in the supernatants was measured using a cytometric bead array (CBA) or ELISA. Allogeneic T cells were stimulated using cells from the cultures, or by CD3/CD28 ligation together with culture supernatants. The proliferation and the phenotype of the T cells was subsequently evaluated.