Figure 4.

Cytokine secretion profiles in the natural killer (NK)–dendritic cell (DC) cross talk assay. DCs and NK cells were kept in single cultures, or in cross talk cultures consisting of NK cells and DCs from the same donor at a 1:1 ratio and exposed to 1 μg/ml F-HIV, complement-opsonized HIV (C-HIV), CI-HIV, or mock treated for 24 h. The experiment was replicated four times using cells derived from four different donors. (A) The supernatants were harvested, and the concentration of cytokines was determined using a cytometric bead array or ELISA. The values were normalized (centered) and a heat map was created. (B) Cytokine concentration in the NK–DC cross talk cultures were normalized to the mock treated samples. Boxplots show mean ± SEM.