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. 2018 Apr 30;9:899. doi: 10.3389/fimmu.2018.00899

Figure 5.

Figure 5

HIV decreased the cytotoxicity in natural killer (NK) cells activated by cross talk between NK cells and dendritic cells (DCs). NK cells were maintained in single NK cell cultures, or in cross talk cultures consisting of NK cells and DCs from the same donor at a 1:1 ratio and exposed to 1 μg/ml F-HIV, complement-opsonized HIV (C-HIV), CI-HIV, PHA, or mock treated for 24 h. The experiment was replicated four times using cells derived from four different donors. (A) NK cells from single or purified from the cross talk assays were added to target cells (CFSE-stained K562 cells) at a 1:8 ratio for 6 h and the amount of killed cells were assessed using flow cytometry. (B) Representative histograms for the different experimental conditions. (C) A similar assay was performed, using CFSE-stained DCs as target cells. Boxplots show mean with Tukey error bars. One-way ANOVA followed by a Bonferroni posttest was used to test for statistical significance (*p < 0.05, N = 4).