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. 2018 Apr 30;12:281. doi: 10.3389/fnins.2018.00281

Figure 3.

Figure 3

Overexpression of DGCR8 decreases the generation of deep-layer neurons (A–D) Immunostaining for TBR1 (A, green) or SOX5 (C, green) and mCherry+ electroporated cells (A,C, red) and merged images on coronal cryosections through the dorsal telencephalon of Control and DGCR8 OE mouse embryos at E14.5 after IUEp at E12.5, and quantification of the proportion of TBR1+mCherry+ (B) or SOX5+mCherry+ (D) cells expressed in % over total mCherry+ cells. NL: neuronal layer; scale bar: 20 μm. Error bars indicate the variation of four Control and five DGCR8 OE electroporated cortices (s.e.m.); unpaired Student's t-test. E,F) Western blot and quantification of SOX5 [four (Control) and five (DGCR8 OE) independent pools] in E14.5 Control (white bar) and DGCR8 OE (black bar) mouse dorsal telencephalon after IUEp at E12.5. Values are normalized on ACTIN levels. Error bars indicate the variation of four Control and four DGCR8 OE independent pools (s.e.m.); each independent pool consists of four to five dissected electroporated cortical areas; unpaired Student's t-test.