Figure 7.

Overexpression of DGCR8 does not change composition or functions of the “miRNA-independent Microprocessor” (A–C) Western blot (A) and quantification (B,C) of DROSHA, DGCR8, FOXG1, DDX5 and TDP-43, in lysate (INPUT) or lysate after co-immunoprecipitation (co-IP) with DROSHA, or MOCK immunoprecipitation, from E14.5 Control (white bars, in B, or white-gray-striped bars, in C) and DGCR8 OE (black bars, in B, or black-gray-striped bars, in C) mouse dorsal telencephalon after IUEp at E12.5. Samples were normalized over GAPDH for input samples and normalized to DROSHA for co-immunoprecipitation, error bars indicate the variation of four Control and four DGCR8 OE independent pools (s.e.m.); each independent pool consists of five to six dissected electroporated cortical areas; unpaired Student's t-test. (D) Immunostaining for NGN2 (green) and mCherry+ electroporated cells (red) on coronal cryosections through the dorsal telencephalon of Control and DGCR8 OE mouse embryos at E14.5 after IUEp at E12.5. SVZ: subventricular zone and VZ: ventricular zone; scale bar: 20 μm. (E) Quantification of the proportion of NGN2+mCherry+ cells expressed in % over total mCherry+ cells; Error bars indicate the variation of four Control and four DGCR8 OE electroporated cortices (s.e.m.); unpaired Student's t-test. *p-value < 0.05.