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SHAPE analysis at the U, I, and F conditions. (a) Normalized SHAPE reactivities are shown at 10 mM [Mg2+] + 50 mM [Na+] (top), 0.5 mM [Mg2+] + 50 mM [Na+] (middle), and 25 mM [Na+] (bottom). (b–d) Color-coded regional map is given of the (b) F, (c) I, and (d) U conformations. To see this figure in color, go online.
