Table 1.
List of primers used for genotyping of the mouse models
| Mice | Forward | Size (bp) |
|---|---|---|
| Cre-upstream | F 5′ TTCCCTGGTCCCTATGTCAG 3′ | 1112 |
| R 5′ GTCCCTGAACATGTCCATCA 3′ | ||
| Cre-downstream | F 5′ GTCCATCCCTGAAATCATGC 3′ | 990 |
| R 5′ CTCATTGCCACTGGAAACCT 3′ | ||
| Cre (founders) | F 5′ GAAATGGTTCCCTGCTGAAC 3′ | 600 |
| R 5′ TCTCTGCCCAGAGTCATCCT 3′ | ||
| Prokr2-Cre (progeny) | F 5′ TCCCCACGGTAGTTGTGAAG 3′ | Cre: 353 |
| R 5′ ATTGGTTGGTGTGGTTTGCAG 3′ | Wt: 499 | |
| mt 5′ CAGCAGGTTGGAGACTTTCCT 3′ | ||
| Genomic iCre (qPCR) | F 5′ CCACATTGGCAGGACCAAGA 3′ | 163 |
| R 5′ GGCAGCCACACCATTCTTTC 3′ | ||
| Genomic Il2 (qPCR) | F 5′ CTAGGCCACAGAATTGAAAGATCT 3′ | 324 |
| R 5′ GTAGGTGGAAATTCTAGCATCATCC 3′ | ||
| R26-tdToma | F 5′ CTGTTCCTGTACGGCATGG 3′ | 197 |
| R 5′ GGCATTAAAGCAGCGTATCC 3′ | ||
| R26-GFPa | F 5′ AAGTTCATCTGCACCACCG 3′ | 176 |
| R 5′ TCCTTGAAGAAGATGGTGCG 3′ |
a
Mouse lines are commercially available at JAX® mice