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. Author manuscript; available in PMC: 2018 May 7.
Published in final edited form as: Biochemistry. 2015 Jul 6;54(27):4216–4225. doi: 10.1021/acs.biochem.5b00444

Figure 5.

Figure 5

Kinase activity of EGFR is responsible for lateral phosphorylation. + and − indicate the presence and absence of EGF ligand. Lanes from left to right, WT EGFR (−), WT (+), R776H (−), R776H (+), D855G (−), D855G (+), R776H/D855G (−), R776H/D855G (+), D855G and L704N/V948R (−), D855G and L704N/V948R (+), R776H/D855G and L704N/V948R (−), R776H/D855G and L704N/V948R (+), Y1197F and R776H/V948R (−), Y1197F and L704N/V948R (+), R776H/Y1197F and R776H/V948R (−), R766H/Y1197F and L704N/V948R (+) L704N/V948R is FLAG tagged. All other constructs are GFP tagged.