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. 2018 Apr 25;114(8):1921–1935. doi: 10.1016/j.bpj.2018.03.014

Figure 4.

Figure 4

Single-dye fluorescence measures the partition coefficient between Ld and Lo phases in bSM mixtures. The fluorescence emission (arb. u.) of TOE is measured along a thermodynamic tieline of (A) macrodomain bSM/DOPC/chol (ρ = 1) and (B) nanodomain bSM/POPC/chol (ρ = 0). (C) The fluorescence emission (arb. u.) of Bodipy-PC monitored along tielines in the lipid mixture bSM/DOPC/POPC/chol is shown at different ρ values as described in the text. The profiles are vertically shifted for clarity. Mixtures at ρ = 0.75 and ρ = 0.4 form macro- and nanodomains, respectively. Fits of the experimental data (lines) to Eq. 3 were used to obtain probe Kp in each system, shown in Table 1. To see this figure in color, go online.