Table 1.
Partition Coefficients of Bodipy-PC and TOE
| Lipid Mixture | Dye | Single-Dye Fluorescence | FRET (with DHE) | FRET (with Bodipy-PC) | Microscopy (GUVs) |
|---|---|---|---|---|---|
| DSPC/DOPC/chol | Bodipy-PC | 10 ± 2 | 11 ± 2 | NA | 15 ± 4 |
| DSPC/POPC/chol | Bodipy-PC | 4 ± 1 | ∗ | NA | NA |
| DSPC/DOPC/chol | TOE | 9 ± 1 | 9 ± 2 | 7 ± 1 | NA |
| DSPC/POPC/chol | TOE | 5 ± 1 | ∗ | ∗ | NA |
| bSM/DOPC/chol | Bodipy-PC | 9 ± 2 | 8 ± 1 | NA | 10 ± 2 |
| bSM/DOPC/POPC/chol | Bodipy-PC | 8 ± 2 | 6 ± 1 | NA | 9 ± 2 |
| bSM/DOPC/POPC/chol | Bodipy-PC | 5 ± 1 | ∗ | NA | NA |
| bSM/ POPC/chol | Bodipy-PC | 3 ± 1 | ∗ | NA | NA |
| bSM/DOPC/chol | TOE | 8 ± 1 | 10 ± 2 | 8 ± 1 | NA |
| bSM/POPC/chol | TOE | 5 ± 1 | ∗ | ∗ | NA |
Kps of Bodipy-PC and TOE measured for different lipid mixtures, using single-dye fluorescence, FRET and fluorescence microscopy. Errors in Kp by single-dye fluorescence and FRET measurements are standard error of the free parameter calculated from nonlinear regression analysis of Eqs 3 and 4, respectively. Errors in Kp using fluorescence microscopy correspond to the standard error of 7–10 GUV measurements. NA (not available) represents the noninvestigation of Bodipy-PC homo-FRET or the limitation in measuring Kp using a microscope. The symbol (∗) marks the FRET profiles used in the domain size investigation.