Table 3. Mito–mito epistasis (V_AA) estimates.

Media:		SOE		YPD		YPEG
Temperature (°C)		30	37	30	37	30	37
All crosses	VAA	0.0051	0.0360	0.0011	0.0028	0.0019	0.0042
	C.I. lower	0.0046	0.0354	0.0009	0.0026	0.0017	0.0041
	C.I. upper	0.0078	0.0400	0.0014	0.0032	0.0022	0.0044
Single representation	VAA	0.0112	0.0351	0.0010	0.0030	0.0010	0.0037
	C.I. lower	0.0109	0.0345	0.0008	0.0027	0.0008	0.0036
	C.I. upper	0.0141	0.0394	0.0014	0.0033	0.00144	0.0039
Double representation	VAA	0.0060	0.0413	0.0034	−0.0030	0.0030	0.0037
	C.I. lower	0.0045	0.0395	0.0033	−0.0033	0.0029	0.0035
	C.I. upper	0.0091	0.0473	0.0037	−0.0027	0.0033	0.0038

Positive (nonzero) V_AA estimates indicate mito–mito epistasis. The overall magnitudes of the V_AA values do not provide information regarding their relative contribution to genetic variance. All estimates were significantly different from zero based on permutation analysis (see Materials and Methods). Positive estimates were confirmed based on upper and lower confidence intervals (C.I.s) that did not overlap zero estimated from bootstrapping analysis. Subsets of the data were analyzed for single representation (YJM975 × Y12 and YPS606 × Y55 crosses) or double representation (each mitotype represented twice, YJM975 × Y12 excluded). SOE, ; YPEG, ; YPEGM, .