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. 2018 Mar 20;209(1):129–141. doi: 10.1534/genetics.118.300809

Figure 1.

Figure 1

Checkpoint mutations permit growth of dna2∆ cells at 20°. Diploids heterozygous for dna2∆ and pif1∆, rad9∆, ddc1∆, chk1∆, rad17∆, mec1∆ sml1∆, tel1∆, rad53∆ sml1∆ or sml1∆ mutations were sporulated, tetrads were dissected, and spores germinated. Germination plates were incubated for 10–11 days at 20°, or 3–4 days at 30°. Strains of dna2∆ yfg∆ background are indicated by yellow arrows, and strains of yfg∆ background are indicated by blue arrows. Additional images of growth at 20, 23, or 30° are in Figure S1 in File S1. Strains were as follows: DDY1285, DDY874, DDY876, DDY878, DDY880, DDY958, DDY950, DDY947, DDY952, and DDY1276. Strain details are in Table S1 in File S1.