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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: Bioconjug Chem. 2018 Mar 23;29(4):1291–1301. doi: 10.1021/acs.bioconjchem.8b00058

Figure 4. CSANs are Readily Installed on Cells Modified with Phospholipid Conjugates.

Figure 4

For all panels, cells were labeled with CSANs through one of several methods and then analyzed by flow cytometry with staining for surface-bound nanorings via either an anti-FLAG phycoerythrin or anti-MYC Alexa Fluor 647 conjugate. The non-specific binding of the antibody-fluorophore conjugate is shown in grey, while the specific detection of the indicated CSAN is shown in color. (A) mSA CSANs bind to MCF-7 cells modified with DSPE-PEG2000-biotin. (B) Fn3 CSANs formed with azide-bisMTX are conjugated to Raji cells modified with DSPE-PEG2000-DBCO. (C) Fn3 CSANs bind to unmodified, EpCAM+ MCF-7 cells. (D) Bispecific mSA/Fn3 CSANs bind to Raji cells modified with DSPE-PEG2000-biotin. (E) Bispecific mSA/Fn3 CSANs bind to unmodified, EpCAM+ MCF-7 cells. All experiments were performed in triplicate, with a representative histogram shown for each scenario.