Figure 1.

High concentration hydrogen (HCH) reduces infarct ratio in mice with cerebral ischemia/reperfusion (I/R) injury.

Note: (A) Monitoring of cerebral blood flow (CBF); (B) 2,3,5-triphenyltetrazolium hydrochloride (TTC) staining of brain sections and quantification of infarct ratio (n = 8 per group). After 1-hour ischemia and 24-hour reperfusion, TTC staining and determination of infarct ratio were done in mice. Results showed 90-minute HCH inhalation since the beginning of reperfusion significantly reduced infarct ratio in mice with I/R injury, but the neurological function and infarct ratio were comparable between I/R group and I/R + nitrogen/oxygen (NO) group. Data are expressed as the mean ± SEM, and analyzed by one-way analysis of variance followed by Turkey test. ^###P < 0.001, vs. I/R group. HO: I/R + hydrogen/oxygen group; min: minute(s).