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. 2018 Apr 30;9:878. doi: 10.3389/fimmu.2018.00878

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Copyright © 2018 Zhang, Huang, Li, Dang, Yuan, Wang, Zeng, Sun, Liu, Ao, Tan, Su, Qian, Olsen and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human gingiva-derived mesenchymal stem cells (GMSC) decrease the expression of inflammatory Ly6C^hi monocytes in ApoE^−/− mice. The cells from spleen and draining lymph nodes of ApoE^−/− mice were stained with anti-CD11b, -Ly-6C, Gr1, CCR2, and -CX3CR1 mAbs. Living cells were gated to determine percentages of CD11b⁺, the subsets of Ly-6C^hiCX3CR⁺ in CD11b⁺ cells, CD11b⁺Ly6C^hi, CD11b⁺CCR2⁺, and CD11b⁺Gr1^hi. (A,B,D) Representative dot plots of individual mice from each group were depicted. (C,E) Bar graphs show the mean ± SD (model, n = 6; fibroblast, n = 3; p-GMSC, n = 5; t-GMSC, n = 7). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001.