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. 2018 Mar 12;90(7):4635–4640. doi: 10.1021/acs.analchem.7b05157

Figure 1.

Figure 1

Effect of the charged residues on peptide retention in hSAX. (a) Mean residue count per peptide for D/E (red) and K/R (blue) over fraction. Error bars denote the standard deviation. Peptide count per fraction is shown in orange (total 59 297 unique peptides). (b) Effect of D/E count (range 0–5) on peptide retention. (c) Extracted chromatogram of peptides with three and four D/E (red). Subpopulations were defined according to the number of K/R residues (one to three, blue tones for peptides with three D/E residues and green tones for peptides with four D/E residues). Crosses mark the mode of the respective distributions.