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. 2018 Apr 22;2018:4043726. doi: 10.1155/2018/4043726

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Copyright © 2018 Theresia Zuleger et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The publication of this article was funded by Max Planck.

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LC3-II and ATG12-ATG5 abundances are unaltered in the mouse kidney of Sgk1 knockout mice. LC3 lipidation (a) and ATG12-ATG5 (b) abundances were analysed by Western blotting (upper panel) and quantified (lower panels) using Sgk1 knockout (Sgk1−/−) and wild-type (Sgk1+/+) mice (n = 6). Of those, mice were either fed (F, n = 3) or starved (S, n = 3) as indicated (sample ID). LC3-positive control (Ctrl) was used to indicate the migration of nonlipidated LC3-I and lipidated LC3-II. Red lines on the box plots (lower panel, left) represent the mean values of all 6 Sgk1 knockout (Sgk1−/−) and wild-type (Sgk1+/+) mice (lower panel, left) or with regard to fed and starved conditions (lower panel, right). p values are provided. Supporting data is provided (Suppl. Data Set).