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. 2018 Apr 22;2018:4043726. doi: 10.1155/2018/4043726

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Copyright © 2018 Theresia Zuleger et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The publication of this article was funded by Max Planck.

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SGK1 silencing provokes an increase of GFP-WIPI1 puncta. Stable GFP-WIPI1 expressing U-2 OS cells were transiently transfected with control siRNA (siControl) or siSGK1 and fed or starved for 3 h with or without bafilomycin A1 (BafA1). (a) Representative images for starved conditions with and without BafA1 treatment are shown. Scale bars 40 μm. (b) The percentage GFP-WIPI puncta cells were calculated (up to 1541 cells per condition, n = 3). p values are provided. Supporting data is provided (Suppl. Data Set).