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Increased ULK1 protein abundance in SGK1-depleted cells. Stable GFP-WIPI1 expressing U-2 OS cells were transiently transfected with control siRNA (siControl) or siSGK1 and fed or starved for 3 h with or without bafilomycin A1 (BafA1). (a) Phosphorylated and total ULK1 protein was detected by sequential Western blotting. (b) Phospho-ULK1 (S758)/ULK1 and ULK1/tubulin ratios were quantified (n = 3, in duplicates). p values are provided. Supporting data is provided (Suppl. Data Set).
