Skip to main content
. 2018 Apr 23;2018:5346272. doi: 10.1155/2018/5346272

Figure 5.

Figure 5

Involvement of PPAR-γ in the MEKT1 effects on Pomc mRNA expression. Effects of PPAR-γ knockdown by its siRNA on (a) PPAR-γ mRNA expression, (b) PPAR-α mRNA expression, and (c) PPAR-β mRNA expression. AtT20 cells transiently transfected with siRNA (negative control; NC or PPAR-γ) for 48 hours were incubated with 0.1% DMSO (control) for 24 hours. Results are expressed as percentages of each control. Each point represents mean ± SEM (n = 4). ∗P < 0.05 versus basal negative control siRNA. NS stands for “not significant.” (d) Effects of PPAR-γ knockdown by its siRNA on the PPAR-γ protein expression. (e) Effects of PPAR-γ knockdown by its siRNA on the Pomc mRNA expression. AtT20 cells transiently transfected with siRNA (negative control; NC or PPAR-γ) for 48 hours were incubated in the presence of either MEKT1 (10 μM) or 0.1% DMSO (control) for 24 hours, respectively. Data are expressed as percentages (100%) of control. Each point represents mean ± SEM (n = 4). NS stands for “not significant.” ∗P < 0.05 versus negative control siRNA at 10 μM MEKT1.