Skip to main content
. 2018 Apr 22;2018:3654095. doi: 10.1155/2018/3654095

Figure 6.

Figure 6

DPHC protects MGO-induced protein glycation in HEK cells. HEK cells were incubated with or without 40 μM DPHC for 1 h and then further incubated with or without 1 mM MGO for 24 h. (a) AGE content was measured. (b) AGE receptor (RAGE) mRNA expression level was analyzed by qRT-PCR. (c) Protein carbonyl content was measured. (d) Glo-1 mRNA expression level was analyzed by qRT-PCR. (e–i) HEK cells were transfected with Glo-1 siRNA for 36 h and incubated with or without 40 μM DPHC for 1 h and then further incubated with or without 1 mM MGO for 24 h. (e) Glo-1 mRNA expression level was analyzed by qRT-PCR. (f) AGE content was measured. (g) Protein carbonyl content was measured. (h) Glo-1 (glyoxalase 1) expression was measured by qRT-PCR. (i) Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) mRNA expression was measured by qRT-PCR. Experiments were performed in triplicate. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. n.s. indicates no significance.