Table 2. Results of the permutational analysis of variance (PERMANOVA) of the rarefied MOTU richness and Shannon diversity values for the two genes studied.
Post-hoc pairwise tests for the significant main factor fraction are indicated. Pairwise tests for the community factor, when significant, are presented graphically in Figs. 1 and 2.
| df | SS | MS | Pseudo-F | p-value | post-hoc test | |
|---|---|---|---|---|---|---|
| 18S MOTU richness | ||||||
| Site | 1 | 3.57E+04 | 3.57E+04 | 0.72305 | 0.432 | |
| Fraction | 2 | 3.54E+05 | 1.77E+05 | 43.712 | 0.001 | A < B < C |
| Site*fraction | 2 | 3,411.1 | 1,705.6 | 0.42163 | 0.634 | |
| Community (site) | 6 | 2.97E+05 | 49,539 | 15.711 | 0.001 | |
| Sample (community) | 16 | 50,559 | 3,160 | 1.6166 | 0.121 | |
| Residual | 31 | 60,594 | 1,954.6 | |||
| 18S Shannon diversity | ||||||
| Site | 1 | 3.421 | 3.421 | 2.7364 | 0.134 | |
| Fraction | 2 | 8.9238 | 4.4619 | 17.815 | 0.002 | A < B = C |
| Site*fraction | 2 | 0.13855 | 6.93E−02 | 0.27659 | 0.765 | |
| Community (site) | 6 | 3.0166 | 0.25138 | 2.154 | 0.045 | |
| Sample (community) | 16 | 5.078 | 0.31738 | 2.7195 | 0.007 | |
| Residual | 31 | 3.6178 | 0.1167 | |||
| COI MOTU richness | ||||||
| Site | 1 | 18,869 | 18,869 | 0.41923 | 0.523 | |
| Fraction | 2 | 1.70E+06 | 8.51E+05 | 5.78E+00 | 0.026 | A = B < C |
| Site*fraction | 2 | 3.39E+05 | 1.70E+05 | 1.153 | 0.336 | |
| Community (site) | 6 | 2.65E+05 | 4.42E+04 | 1.0125 | 0.495 | |
| Sample (community) | 16 | 6.84E+05 | 4.28E+04 | 0.66873 | 0.864 | |
| Residual | 28 | 1.79E+06 | 6.39E+04 | |||
| COI Shannon diversity | ||||||
| Site | 1 | 1.6876 | 1.6876 | 2.7634 | 0.117 | |
| Fraction | 2 | 11.172 | 5.5859 | 11.034 | 0.004 | A = B < C |
| Site*fraction | 2 | 0.35745 | 0.17872 | 0.35305 | 0.702 | |
| Community (site) | 6 | 3.7634 | 0.62724 | 5.1615 | 0.004 | |
| Sample (community) | 16 | 1.881 | 0.11756 | 0.56391 | 0.892 | |
| Residual | 28 | 5.8373 | 0.20847 |