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. 2018 May 1;11:2475–2487. doi: 10.2147/OTT.S160192

Figure 6.

Figure 6

UCA1 acted as a ceRNA of miR129 to enhance target SOX4 gene expression in RCC cells.

Notes: (A) 786O cells were transfected with miR129 mimic or miR-Con, and ACHN cells were transfected with miR129 inhibitor or anti-miR-Con, followed by the detection of SOX4 protein expression. (B) 786O cells were transfected with siCon or siUCA1-2, and ACHN cells were transfected with pcDNA3.1 vector or pcDNA-UCA1 plasmid, followed by the determination of SOX4 protein expression. (C) Effects of miR129 and UCA1 overexpression on luciferase activity of SOX4 reporter were evaluated in 786O cells. (D) The effect of miR129 downregulation and UCA1 silencing on luciferase activity of SOX4 reporter was monitored in ACHN cells. (E) SOX4 mRNA expression in 40 pairs of RCC tumor tissue and adjacent normal tissue. (F) Spearman’s correlation analysis of SOX4 mRNA and UCA1 expressions in 40 cases of RCC tumor tissue. *P<0.05.

Abbreviation: RCC, renal cell carcinoma.