Fig 10.
Effect of TCDD on mRNA expression of BA regulators and transporters in ilea of WT male (blue bars) and female (red bars) mice. Corn oil (vehicle) or TCDD (37 μg/kg) was administered daily (IP) for 4 days to male and female mice (at least 6 mice per treatment group). On the 5th day ilea were harvested. Total RNA was analyzed by QuantiGene Plex 2.0 Assay, as well as by RT-qPCR. Relative mRNA levels were calculated with vehicle controls set as 100%. Bars represent the relative percentage mRNA expression ± SE of mice per group. Asterisks indicate significant difference (p < 0.05) from the respective value of the WT. ATP-binding cassette (Abc), Apical sodium-dependent bile acid transporter (Asbt), Farnesoid X Receptor (Fxr), females (F), Fibroblast growth factor (Fgf), I-babp (ileal bile acid binding protein), Liver x receptor a (Lxra), males (M), Multidrug resistance-associated protein (Mrp), Nieman-Pick c1-like 1 (Npc1l1), Organic solute transporter (Ost), total bile acids (Σ-BAs), Small heterodimer partner (Shp), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), Transmembrane G protein-coupled receptor 5 (Tgr5), wild-type mice (WT). Color image is available in the online version of the article.