Fig. 6.
Effect of TCDD on serum concentration of total bile acids (top), individual T-conjugated bile acids (middle), and individual unconjugated bile acids (bottom) in WT male (blue bars) and female mice (red bars). Corn oil (vehicle) or TCDD (37 μg/kg) was administered daily (IP) for 4 days to male and female mice (at least 6 mice per treatment group). On the 5th day blood was collected and the individual BAs were quantified in sera by UPLC-MS/MS. Bars represent the mean ± SE of mice per group. Asterisks indicate significant difference (p < 0.05) from the respective value of the WT mice. Primary bile acids (1°BAs), secondary bile acids (2°BAs), 6-hydroxylated bile acids (6-OH), 12α-hydroxylated (12-OH) bile acids, cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), females (F), hyodeoxycholic acid (HDCA), lithocholic acid (LCA), males (M), muricholic acid (MCA), Non 6-, non-12α-hydroxylated bile acids (non-6,12-OH), total bile acids (Σ-BAs), T-conjugated bile acids (T-BAs), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), unconjugated bile acids (U-BAs), ursodeoxycholic acid (UDCA), wild-type mice (WT). Color image is available in the online version of the article.