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TREC imaging: UCP1 distribution and functionality. (a) Topography image. UCP1 molecules imaged with an ATP-tethered tip. (b) Corresponding recognition image. Black dots of the recognition image arise from the decrease of the oscillation upwards peaks as a result from UCP1-ATP binding during recognition. (c) Superposition of recognition map of UCP1-ATP binding domains (in pink) onto the corresponding topography image. Color scale (green to pink) is 0–8 nm. Image sizes are 600 × 600 nm.
