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. Author manuscript; available in PMC: 2018 May 7.
Published in final edited form as: Free Radic Biol Med. 2016 Mar 22;95:293–307. doi: 10.1016/j.freeradbiomed.2016.03.011

Figure 2. 13C NMR spectrum (A) and identified glycated PLPE (B).

Figure 2

A - 13C pulse experiment (i.e. without 1H decoupling) of the Amadori products built upon reaction of 1,2-13C2-Glucose with PLPE (top). Resonances of four Amadori products were identified and simulations were fitted to the experimental spectrum (bottom). The 13C heptett of the CD3OD solvent was referenced to 48.5 ppm but truncated at the top. B - Possible conformations of Amadori products identified in the NMR spectrum: (i) α-1-(N-PLPE)-d-glucopyranose, (ii) α-1-(N-PLPE)-d-glucofuranose, (iii) β-1-(N-PLPE)-d-glucopyranose, and (iv) β-1-(N-PLPE)-d-glucofuranose. Only two rotamers are shown for the α- and β-glucopyranose forms.