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The average percentage of production of prodeoxyviolacein 3 by each mutant relative to that of wild-type VioE is shown, with error bars representing the standard deviation over three trials. Y17A, S19A, N51A, E66A, and R172A show significantly reduced prodeoxyviolacein 3 production relative to wild type, whereas C35A, M64A, K77A, and S170A produce prodeoxyviolacein 3 at a level that is not significantly different from wild type.
