Table 1.
Manually validated ASK1 phosphorylation sites identified by shotgun and PRM MS.
| Status1 | A.A. site | Stimulation | Identified sequences |
|---|---|---|---|
| Known | S82/83 | Both | GSSVGGGSRR |
| Known | S82/83 | Both | GRGSSVGGGSR |
| Known | S82/83 | Both | GRGSSVGGGSRR |
| Known | S82/83 | H2O2 | GSSVGGGSR |
| Novel | T140/141 | HNE | LDFGETTVLDR |
| Novel | S268/269 | H2O2 | VAQASSSQYFR |
| Novel | Y355 | H2O2 | FHYAFALNR |
| Known | T947/S952 | HNE | KKKTQPKLSALSAGSNEYLR |
| Known | T947/S952 | Both | KKTQPKLSALSAGSNEYLR |
| Novel | S955 | Both | LSALSAGSNEYLR |
| Known | S958 | Both | LSALSAGSNEYLR |
| S955/S958 | HNE | TQPKLSALSAGSNEYLR | |
| S955/S958 | HNE | KTQPKLSALSAGSNEYLR | |
| Novel | T1000/S1004 | HNE | TRAKSCGERDVKGIR |
| Novel | T1000/S1004 | HNE | TRAKSCGERDVK |
| Novel | S1004 | Both | AKSCGERDVK |
| Known | T1059 | H2O2 | ILTEDQDKIVR |
| Novel | S1240 | HNE | SLNVQLGR |
1
Known=previously reported site, Novel=unreported phosphorylation site