Maximal depolarizations of alfalfa root hairs in response to 0.1 μm NodRm-IV(C16:2,S), measured in different Ca2+ solutions (pCa) with and without EGTA, as indicated. Points are from single experiments (○, ♦) or mean values (•, ±se); numbers in brackets refer to the number of experiments. Shaded area compares the effect of NodRm-IV(C16:2,S) on membrane depolarization in the presence of 0 (•), 0.1 (♦), and 0.01 (○) mm EGTA at equal free [Ca2+].